Composite

Part:BBa_K1765000:Design

Designed by: Kinuko Ueno   Group: iGEM15_Tokyo-NoKoGen   (2015-09-06)


Pconst(low)-G3DH-T0 terminator-Pconst(low)-OtsB-OtsA-T0 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2409
    Illegal NheI site found at 2899
    Illegal NheI site found at 2922
    Illegal NotI site found at 390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2715
    Illegal BglII site found at 3426
    Illegal BamHI site found at 4181
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 630
    Illegal NgoMIV site found at 1846
    Illegal NgoMIV site found at 2142
    Illegal NgoMIV site found at 2695
    Illegal NgoMIV site found at 2760
    Illegal AgeI site found at 812
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1903
    Illegal SapI site found at 2007


Design Notes

This parts comes from previous parts, BBa_K1339018 and BBa_K1339024.


Source

It comes from genomic sequence of E.coli, DH5α.

References